Benefits

Superior Quality

The human Multimeric Adiponectin ELISA system was developed for the selective quantification of human adiponectin oligomers using protease pretreatment(1), and the mouse Adiponectin ELISA reflects a similar system for simultaneous detection of both mouse high-molecular-weight (HMW) and total adiponectin(2).

The human Multimeric Adiponectin ELISA from ALPCO and Sekisui has been cited in numerous publications, and is trusted by the research community worldwide for its consistency and quality. The new mouse HMW and Total Adiponectin ELISA has been held to the same exacting standards of quality in research, development and pre-market testing.

Unique

Other ELISA methods on the market do not allow for simultaneous testing of both HMW and total adiponectin. In fact, other commercial methods for mouse HMW adiponectin lack a corresponding method for the assessment of total adiponectin. In the absence of an international reference standard for adiponectin, the determination of both HMW and total adiponectin using the same ELISA is preferable to eliminate this source of bias when calculating the HMW/total ratio.

Cost-Effective

Adiponectin oligomers in mice have, until now, been quantified only by using time-intensive, multi-step procedures which are prohibitive for many laboratories. The ALPCO ELISA offers researchers a straight-forward sample pre-treatment protocol and simple ELISA procedure, and allows measurement of HMW and total adiponectin in the same 96-well plate, eliminating between day or between assay bias. The ELISA is an affordable option which does not sacrifice reliability or accuracy.

Minimal Sample Commitment

Mouse sample volumes are low, and the list of interesting biomarkers grows longer with each passing scientific congress and convention! The development teams at ALPCO and Sekisui understand these constraints, and recognize that our assays must operate within them. This ELISA requires only a total of 20 µl of sample to measure both HMW and Total adiponectin in duplicate. It is possible, supported by in-house data published in the instructions for use, to reduce the total volume of sample required. We suggest that you speak with ALPCO representatives or your distributor for details on using a smaller sample volume.

  1. 1. Ebinuma, H et al. Clinica Chimica Acta 2006; 372: 47-53.
  2. 2. Ebinuma, H et al. Clinica Chimica Acta 2009; 401: 181-183.